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Pedigree relationships were verified and likely genotyping errors were detected using PREST software version 3.02 (Pedigree Relationship Statistical Tests, (Mc Peek and Sun 2000; Sun et al. The median [interquartile range (IQR)] percentages of urine arsenic species were reported for the overall population and according to age (≤ 55 years, ), smoking (never, former, current), alcohol consumption (never, former, current), and study region (Arizona, Oklahoma, and North and South Dakota). We estimated the heritability of %i As, %MMA, and %DMA using a general pedigree variance components decomposition-based method as conducted by the software Sequential Oligogenic Linkage Analysis Routines (SOLAR) version 4.4.0 (Almasy et al. For a detailed description of the statistical methods used to estimate heritability, see Supplemental Material, pp. In brief, SOLAR incorporates the information contained in participant pedigrees to obtain maximum likelihood estimates for the proportion of unexplained variance due to additive genetic effects from polygenes (σ is constrained to be zero, following a 1/2:1/2 mixture of chi-square distributions with 1 degree of freedom (df) and a point mass at zero (Amos 1994). sh RNA silencing of AS3MT expression minimizes arsenic methylation capacity of Hep G2 cells. … continue reading »

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